In 2015, the Advanced Resistive Exercise Device (ARED) foot panel on the International Space Station (ISS) was sampled to identify culturable microorganisms. The whole-genome of the strain IF3SW-P1 was sequenced using the Oxford Nanopore Technologies platform and identified as Klebsiella quasipneumoniae subsp. quasipneuomoniae, a Biosafety Level-2 (BSL-2) human pathogen. This whole-genome sequence will aid in comparative genomic analyses with Earth-origin pathogens for differences in virulence and antimicrobial resistance in microorganisms exposed to spaceflight conditions.