mRNA expression profile in DLD-1 and MOLT-4 cancer cell lines cultured under Microgravity

DLD-1 and MOLT-4 cell lines were cultured in a rotating cell culture system to simulate microgravity and mRNA expression profile in comparison to Static controls. Cells were grown in 10mL rotating vessels in a Rotary Cell Culture System (RCCS) and in 60mm Petri dishes (test control respectively). Two replicates of test (Microgravity) and control (static) each from DLD-1 and MOLT-4 were analyzed by microarray. Simulated microgravity affected the solid tumor cell line DLD-1 markedly which showed a higher percentage of dysregulated genes compared to the hematological tumor cell line MOLT-4. Microgravity affects the cell cycle of DLD-1 cells and disturbs expression of cell cycle regulatory gene networks. Multiple microRNA host genes were dysregulated and significantly mir-22 tumor suppressor microRNA is highly upregulated in DLD-1.

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Additional Info

Field Value
Maintainer GeneLab Outreach
Last Updated April 23, 2025, 23:02 (UTC)
Created April 1, 2025, 01:11 (UTC)
accessLevel public
accrualPeriodicity irregular
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identifier nasa_genelab_GLDS-125_um5r-gqim
issued 2021-05-21
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modified 2023-01-26
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