mRNA expression profile in DLD-1 and MOLT-4 cancer cell lines cultured under Microgravity

DLD-1 and MOLT-4 cell lines were cultured in a Rotating cell culture system to simulate microgravity and mRNA expression profile was observed in comparison to Static controls. Cells were grown in 10mL rotating vessels in an RCCS and in 60mm Petri dishes (test control respectively).Two replicates of test (Microgravity) and control (static) each from DLD-1 and MOLT-4 were analyzed by microarray. Simulated microgravity affected the solid tumor cell line DLD-1 markedly which showed a higher percentage of dysregulated genes compared to the hematological tumor cell line MOLT-4. Microgravity affects the cell cycle of DLD-1 cells and disturbs expression of cell cycle regulatory gene networks. Multiple microRNA host genes were dysregulated and significantly mir-22 tumor suppressor microRNA is highly upregulated in DLD-1.

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Additional Info

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Maintainer GeneLab Outreach
Last Updated April 23, 2025, 22:48 (UTC)
Created March 31, 2025, 18:59 (UTC)
accessLevel public
accrualPeriodicity irregular
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identifier nasa_genelab_GLDS-125_fway-due9
issued 2018-06-26
landingPage https://data.nasa.gov/dataset/mrna-expression-profile-in-dld-1-and-molt-4-cancer-cell-lines-cultured-under-microgravity
modified 2023-01-26
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