Effect of electromagnetic fields on the chondrogenic differentiation under microgravity conditions

A combination therapy of electromagnetic fields (EMF) and simulated microgravity (SMG) has not been examined in regenerative medicine of cartilage. In the present study a bioreactor system using extremely low-frequency EMF and SMG was applied during the chondrogenic differentiation of human mesenchymal stem cells (hMSCs). It was hypothesized that a beneficial effect of EMF regarding chondrogenesis (COL2A) could be combined with an avoiding effect of SMG regarding hypertrophy (COLXA1) of cartilage. Pellet cultures of hMSCs formed cartilaginous tissue under the addition of growth factors (FGF; TGF-beta3). Pure SMG reduced COLXA1 expression but also COL2A expression of hMSCs. Pure EMF showed no gene expression changes of hMSCs during chondrogenic differentiation. Combining EMF/SMG resulted in a re-increase of COL2A but did not reach control levels. The COL2A to COLXA1 ratio of combined EMF/SMG was not significantly different from control levels. The combination therapy of EMF/SMG did not significantly improve the chondrogenic potential of hMSCs. chondrogenic differentiation electromagnetic stimulation-control 1 timepoint with/without stimulation

Data and Resources

Additional Info

Field Value
Maintainer GeneLab Outreach
Last Updated April 23, 2025, 22:51 (UTC)
Created March 31, 2025, 20:43 (UTC)
accessLevel public
accrualPeriodicity irregular
bureauCode {026:00}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
catalog_@id https://data.nasa.gov/data.json
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
harvest_object_id 0f74a8ab-6812-4234-8ba0-a1422d4ede5c
harvest_source_id 61638e72-b36c-4866-9d28-551a3062f158
harvest_source_title DNG Legacy Data
identifier nasa_genelab_GLDS-124_jiw5-nq97
issued 2018-06-26
landingPage https://data.nasa.gov/dataset/effect-of-electromagnetic-fields-on-the-chondrogenic-differentiation-under-microgravity-co
modified 2023-01-26
programCode {026:005}
publisher National Aeronautics and Space Administration
resource-type Dataset
source_datajson_identifier true
source_hash 41f8fc24ab345a9833d3e22838346cab7f2f794223f2e0d4683cf654aad988b7
source_schema_version 1.1
theme {"Earth Science"}