@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix org: <http://www.w3.org/ns/org#> .
@prefix skos: <http://www.w3.org/2004/02/skos/core#> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://data.nasa.gov/dataset/524d9a82-df2e-4bef-814f-5fedccb0fb84> a dcat:Dataset ;
    dct:description "Accumulating data support the concept that ionizing radiation therapy (RT) has the potential to convert the tumor into an in situ individualized vaccine; however this potential is rarely realized by RT alone. Transforming growth factor  xce xb2 (TGF xce xb2) is an immunosuppressive cytokine that is activated by RT and inhibits the antigen-presenting function of dendritic cells and the differentiation of effector CD8+ T cells. Here we tested the hypothesis that TGF xce xb2 hinders the ability of RT to promote anti-tumor immunity. Development of tumor-specific immunity was examined in a pre-clinical model of metastatic breast cancer. Mice bearing established 4T1 mouse mammary carcinoma treated with pan-isoform specific TGF xce xb2 neutralizing antibody 1D11 showed significantly improved control of the irradiated tumor and non-irradiated metastases but no effect in the absence of RT. Notably whole tumor transcriptional analysis demonstrated the selective upregulation of genes associated with immune-mediated rejection only in tumors of mice treated with RT+TGF xce xb2 blockade. Mice treated with RT+TGF xce xb2 blockade exhibited cross-priming of CD8+ T cells producing IFN xce xb3 in response to three tumor-specific antigens in tumor-draining lymph nodes which was not evident for single modality treatment. Analysis of the immune infiltrate in mouse tumors showed a significant increase in CD4+ and CD8+ T cells only in mice treated with the combination of RT+TGF xce xb2 blockade. Depletion of CD4+ or CD8+ T cells abrogated the therapeutic benefit of RT+TGF xce xb2 blockade. These data identify TGF xce xb2 as a master inhibitor of the ability of RT to generate an in situ tumor vaccine which supports testing inhibition of TGF xce xb2 during radiotherapy to promote therapeutically effective anti-tumor immunity. We used genome-wide microarray to depict main biological processes responsibles for the therapeutic benefit of the combination ofTGF-beta blockade and local radiotherapy. To gain a more comprehensice protrait of the effects of RT and TGFbeta blockade on gene expressionin tumors we collected 4T1 tumors 4 days after completion of RT. Three tumors from each group were then subjected to RNA extraction and hybridization on affymetrix array." ;
    dct:identifier "nasa_genelab_GLDS-160_t729-nms2" ;
    dct:issued "2018-06-26"^^xsd:date ;
    dct:modified "2023-01-26"^^xsd:date ;
    dct:publisher <https://data.nasa.gov/organization/dbb294e4-8597-44f5-aaf4-c0baac12cdbc> ;
    dct:title "Gene expression data from 4T1 irradiated tumors treated with TGFbeta blockade" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "GeneLab Outreach" ;
            vcard:hasEmail <mailto:genelab-outreach@lists.nasa.gov> ] ;
    dcat:distribution <https://data.nasa.gov/dataset/524d9a82-df2e-4bef-814f-5fedccb0fb84/resource/4fc73f77-0834-4e90-9d24-ab662155ba50> ;
    dcat:keyword "array-scanning-protocol",
        "dose",
        "growth-protocol",
        "hybridization-protocol",
        "injection",
        "ionizing-radiation",
        "labelling-protocol",
        "normalization-data-transformation-protocol",
        "nucleic-acid-extraction-protocol",
        "p-gse61208-1",
        "p-gse61208-2",
        "p-gse61208-3",
        "p-gse61208-4",
        "p-gse61208-5",
        "p-gse61208-6",
        "p-gse61208-7",
        "sample-treatment-protocol" ;
    dcat:theme <%7B%22Earth%20Science%22%7D> .

<https://data.nasa.gov/dataset/524d9a82-df2e-4bef-814f-5fedccb0fb84/resource/4fc73f77-0834-4e90-9d24-ab662155ba50> a dcat:Distribution ;
    dct:description "GeneLab Study Page" ;
    dct:format "HTML" ;
    dct:identifier "4fc73f77-0834-4e90-9d24-ab662155ba50" ;
    dct:issued "2025-04-01T00:27:13.944854"^^xsd:dateTime ;
    dct:modified "2025-04-01T00:27:13.891100"^^xsd:dateTime ;
    dct:title "Gene expression data from 4T1 irradiated tumors treated with TGFbeta blockade" ;
    dcat:accessURL <https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-160> ;
    dcat:mediaType "text/html" .

<https://data.nasa.gov/organization/dbb294e4-8597-44f5-aaf4-c0baac12cdbc> a org:Organization ;
    skos:prefLabel "NASA" ;
    foaf:name "NASA" .