@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix org: <http://www.w3.org/ns/org#> .
@prefix skos: <http://www.w3.org/2004/02/skos/core#> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://data.nasa.gov/dataset/076ad492-ea4d-427b-b970-d46415e2b49a> a dcat:Dataset ;
    dct:description "BACKGROUND: Ionizing radiation (IR) can be extremely harmful for human cells since an improper DNA-damage response (DDR) to IR can contribute to carcinogenesis initiation. Perturbations in DDR pathway can originate from alteration in the functionality of the microRNA-mediated gene regulation being microRNAs (miRNAs) small noncoding RNA that act as post-transcriptional regulators of gene expression. In this study we gained insight into the role of miRNAs in the regulation of DDR to IR under microgravity a condition of weightlessness experienced by astronauts during space missions which could have a synergistic action on cells increasing the risk of radiation exposure. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed miRNA expression profile of human peripheral blood lymphocytes (PBL) incubated for 4 and 24 h in normal gravity (1 g) and in modeled microgravity (MMG) during the repair time after irradiation with 0.2 and 2Gy of gamma-rays. Our results show that MMG alters miRNA expression signature of irradiated PBL by decreasing the number of radio-responsive miRNAs. Moreover let-7i* miR-7 miR-7-1* miR-27a miR-144 miR-200a miR-598 miR-650 are deregulated by the combined action of radiation and MMG. Integrated analyses of miRNA and mRNA expression profiles carried out on PBL of the same donors identified significant miRNA-mRNA anti-correlations of DDR pathway. Gene Ontology analysis reports that the biological category of Response to DNA damage is enriched when PBL are incubated in 1 g but not in MMG. Moreover some anti-correlated genes of p53-pathway show a different expression level between 1 g and MMG. Functional validation assays using luciferase reporter constructs confirmed miRNA-mRNA interactions derived from target prediction analyses. CONCLUSIONS/SIGNIFICANCE: On the whole by integrating the transcriptome and microRNome we provide evidence that modeled microgravity can affects the DNA-damage response to IR in human PBL." ;
    dct:identifier "nasa_genelab_GLDS-128_krvn-94zk" ;
    dct:issued "2018-06-26"^^xsd:date ;
    dct:modified "2023-01-26"^^xsd:date ;
    dct:publisher <https://data.nasa.gov/organization/dbb294e4-8597-44f5-aaf4-c0baac12cdbc> ;
    dct:title "Identification of miRNAs involved in cell response to ionising radiation and modeled microgravity" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "GeneLab Outreach" ;
            vcard:hasEmail <mailto:genelab-outreach@lists.nasa.gov> ] ;
    dcat:distribution <https://data.nasa.gov/dataset/076ad492-ea4d-427b-b970-d46415e2b49a/resource/1efb8176-90ad-427e-97d4-e880d5b69b31> ;
    dcat:keyword "data-collection",
        "ionizing-radiation",
        "labeling",
        "normalization-data-transformation",
        "nucleic-acid-hybridization",
        "rna-extraction",
        "sample-collection",
        "simulated-microgravity",
        "timepoint" ;
    dcat:theme <%7B%22Earth%20Science%22%7D> .

<https://data.nasa.gov/dataset/076ad492-ea4d-427b-b970-d46415e2b49a/resource/1efb8176-90ad-427e-97d4-e880d5b69b31> a dcat:Distribution ;
    dct:description "GeneLab Study Page" ;
    dct:format "HTML" ;
    dct:identifier "1efb8176-90ad-427e-97d4-e880d5b69b31" ;
    dct:issued "2025-03-31T21:18:48.322392"^^xsd:dateTime ;
    dct:modified "2025-03-31T21:18:48.294223"^^xsd:dateTime ;
    dct:title "Identification of miRNAs involved in cell response to ionising radiation and modeled microgravity" ;
    dcat:accessURL <https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-128> ;
    dcat:mediaType "text/html" .

<https://data.nasa.gov/organization/dbb294e4-8597-44f5-aaf4-c0baac12cdbc> a org:Organization ;
    skos:prefLabel "NASA" ;
    foaf:name "NASA" .