***An accelerated Life Testing Dataset for Lithium-Ion Batteries with Constant and Variable Loading Conditions***

We present an accelerated Li-ion battery life cycle dataset focused on a large range of load levels and the characterization of the life cycle of a battery pack composed of two 18650 battery cells. The life cycle study is conducted with a total of 26 battery packs that are grouped by constant and random loading conditions, loading levels and number of load level changes. Furthermore, we conducted load cycling on second-life batteries, where surviving cells from previously aged battery packs were assembled to second- life packs.

The dataset was generated from a custom-made testbed to cycle battery packs designed and developed by Kajetan Fricke, Renato Nascimento, and Prof. Felipe Viana, from the Probabilistic Mechanics Laboratory at the University of Central Florida (UCF).

Researchers at NASA GRC conducted an experimental investigation of the flow physics of turbine film cooling by obtaining detailed mean and fluctuating velocity and temperature surveys in the turbulent jet flow field behind a row of large scale film cooling holes. Surface heat transfer film effectiveness and turbulent heat flux values were also determined. Measurements were made in the NASA ERB SW-6 test facility, which consists of an 8.15-inch square open-inlet wind tunnel with a temperature-controlled secondary flow system. The test plate consisted of a three-hole array of film cooling holes that were fed from a plenum of different temperature. The model geometry was scaled up roughly 30 times of typical turbine film cooling size to enable high spatial resolution measurements near the film coolant hole. High-resolution survey data was taken along a centerline streamwise plane behind the hole as well as at various cross-sectional planes behind the hole at two blowing ratios. Mean and fluctuating flow field velocity and temperature measurements were made using various thin wire anemometry probes; surface temperature and heat transfer measurements were made employing liquid crystal and infrared imaging techniques. In addition, a two-plane particle image velocimetry (PIV) technique was performed downstream of the coolant holes to compare with the velocity and turbulent quantities measured by the hotwire probe.

Genome-wide transcriptional profiling shows that reducing gravity levels in the International Space Station (ISS) causes important alterations in Drosophila gene expression. However simulation experiments on ground without space constraints show weaker effects than space environment. A global and integrative analysis using the gene expression dynamics inspector (GEDI) self-organizing maps reveals a subtle response of the transcriptome using different populations and microgravity and hypergravity simulation devices. These results suggest that in addition to behavioural responses that can be detected also at the gene expression level the transcriptome is finely tuned to normal gravity. The alteration of this constant parameter on Earth can have effects on gene expression that depends both on the environmental conditions and the ground based facility used to compensate the gravity vector. Alternative and commons effects of mechanical facilities like the Random Positioning Machine and a centrifuge and strong magnetic field ones like a cryogenically cooled superconductive magnet are discussed. We compare the effects over the gene expression profile of different gender/age Drosophila imagoes in 3-4 days-long experiments under altered gravity conditions into three GBF (Ground Based Facilities for micro/hyper- gravity simulation) using whole genome microarray platforms. Descriptions of different GBFs (treatments): LDC means Large Diameter Centrifuge. Samples can be placed under three conditions: inside LDC (at certain g level) at the LDC rotational control and at external 1g control (outside the LDC). RPM means Random Positioning Machine. Samples can be placed under two conditions: inside RPM (at nearly 0g Microgravity level) and at external 1g control (outside the RPM). At the magnet means INSIDE the Magnetic levitator (another GBF). Samples can be placed under four conditions: inside Magnet 0g* (at microgravity with magnetic field) inside Magnet at 1g* (internal control with magnetic field) or inside the magnet 2g* (at hypergravity with magnetic field) and at external 1g control (outside the magnet)

Genome-wide transcriptional profiling showed that reducing gravity levels in the International Space Station (ISS) causes important alterations in Drosophila gene expression intimately linked to imposed spaceflight-related environmental constrains during Drosophila metamorphosis. However simulation experiments on ground testing space-related environmental constraints show differential responses. Curiously although particular genes are not common in the different experiments the same GO groups including a large multigene family related with behavior stress response and organogenesis are over represented in them. A global and integrative analysis using the gene expression dynamics inspector (GEDI) self-organizing maps reveals different degrees in the responses of the transcriptome when using different environmental conditions or microgravity/hypergravity simulation devices. These results suggest that the transcriptome is finely tuned to normal gravity. In regular environmental conditions the alteration of this constant parameter on Earth can have mild effects on gene expression but when environmental conditions are far from optimal the gene expression is much more intense and consistent effects.

Drosophila samples were exposed to the levitation magnet inside a 25mm diameter tubes with 3 ml of yeast-based Drosophila food in the bottom and a chamber of only 5 mm of height over the food. This small region is needed in order to guarantee that all the flies were located in the effective g area so a maximum of 35 to 40 imagos or pupa can be exposed to each condition per experiment. All experiments were carried out with a parallel 1g external control in a temperature regulated incubator outside the magnet. Three experiments of different duration were performed inside the magnet system to analyze the effect of strong magnetic fields and magnetic levitation during different stages of the Drosophila development

To realistically evaluate the effects of the environment in space it is necessary to understand the effects of external factors during sample transport from Earth to space. The present study focused on temperature profiling the altered gene expression that develops under low cultivation temperatures in C. elegans used as a space life science model. The 7903 genes were selected as differentially expressed genes and divided into five sets with similar expression patterns using k-means clustering. Results from Gene Ontology analysis are significantly indicated that the cell cycle related genes and the TGF?/insulin-like signal pathway related genes changed. The TGF?/insulin-like signal pathway is expected to be activated due to low temperatures as well as by other stressors. To determine the genes whose expression changed four thermal conditions (10 15 20 and 25 xa1C) DNA microarray analysis was performed. The data consisted of 12 samples consisting of three biological replicates at each temperature.

Genome-wide transcriptional profiling shows that reducing gravity levels in the International Space Station (ISS) causes important alterations in Drosophila gene expression. However simulation experiments on ground without space constraints show weaker effects than space environment. A global and integrative analysis using the gene expression dynamics inspector (GEDI) self-organizing maps reveals a subtle response of the transcriptome using different populations and microgravity and hypergravity simulation devices. These results suggest that in addition to behavioural responses that can be detected also at the gene expression level the transcriptome is finely tuned to normal gravity. The alteration of this constant parameter on Earth can have effects on gene expression that depends both on the environmental conditions and the ground based facility used to compensate the gravity vector. Alternative and commons effects of mechanical facilities like the Random Positioning Machine and a centrifuge and strong magnetic field ones like a cryogenically cooled superconductive magnet are discussed. We compare the effects over the gene expression profile of different gender/age Drosophila imagoes in 3-4 days-long experiments under altered gravity conditions into three GBF (Ground Based Facilities for micro/hyper- gravity simulation) using whole genome microarray platforms. Descriptions of different GBFs (treatments): LDC means Large Diameter Centrifuge. Samples can be placed under three conditions: inside LDC (at certain g level) at the LDC rotational control and at external 1g control (outside the LDC). RPM means Random Positioning Machine. Samples can be placed under two conditions: inside RPM (at nearly 0g Microgravity level) and at external 1g control (outside the RPM). At the magnet means INSIDE the Magnetic levitator (another GBF). Samples can be placed under four conditions: inside Magnet 0g* (at microgravity with magnetic field) inside Magnet at 1g* (internal control with magnetic field) or inside the magnet 2g* (at hypergravity with magnetic field) and at external 1g control (outside the magnet)

Drosophila samples were exposed to the levitation magnet inside a 25mm diameter tubes with 3 ml of yeast-based Drosophila food in the bottom and a chamber of only 5 mm of height over the food. This small region is needed in order to guarantee that all the flies were located in the effective g area so a maximum of 35 to 40 imagos or pupa can be exposed to each condition per experiment. All experiments were carried out with a parallel 1g external control in a temperature regulated incubator outside the magnet. Three experiments of different duration were performed inside the magnet system to analyze the effect of strong magnetic fields and magnetic levitation during different stages of the Drosophila development

Genome-wide transcriptional profiling showed that reducing gravity levels in the International Space Station (ISS) causes important alterations in Drosophila gene expression intimately linked to imposed spaceflight-related environmental constrains during Drosophila metamorphosis. However simulation experiments on ground testing space-related environmental constraints show differential responses. Curiously although particular genes are not common in the different experiments the same GO groups including a large multigene family related with behavior stress response and organogenesis are over represented in them. A global and integrative analysis using the gene expression dynamics inspector (GEDI) self-organizing maps reveals different degrees in the responses of the transcriptome when using different environmental conditions or microgravity/hypergravity simulation devices. These results suggest that the transcriptome is finely tuned to normal gravity. In regular environmental conditions the alteration of this constant parameter on Earth can have mild effects on gene expression but when environmental conditions are far from optimal the gene expression is much more intense and consistent effects.

To realistically evaluate the effects of the environment in space it is necessary to understand the effects of external factors during sample transport from Earth to space. The present study focused on temperature profiling the altered gene expression that develops under low cultivation temperatures in C. elegans used as a space life science model. The 7903 genes were selected as differentially expressed genes and divided into five sets with similar expression patterns using k-means clustering. Results from Gene Ontology analysis are significantly indicated that the cell cycle related genes and the TGF?/insulin-like signal pathway related genes changed. The TGF?/insulin-like signal pathway is expected to be activated due to low temperatures as well as by other stressors. To determine the genes whose expression changed four thermal conditions (10 15 20 and 25 xa1C) DNA microarray analysis was performed. The data consisted of 12 samples consisting of three biological replicates at each temperature.