Analysis of effect of hindlimb suspension and reloading on C57Bl/6 mouse soleus muscle. Experimental groups examined: -Control mice 14 days -Hindlimb suspension for 7 days -Hindlimb suspension for 7 days and subsequent reloading for 1 day -Hindlimb suspension for 7 days and subsequent reloading for 7 days

Anoxia induces several heat shock proteins and a heat pre-treatment can acclimatize Arabidopsis seedlings to a subsequent anoxic treatment. In this work we analyzed the response of Arabidopsis seedlings to anoxia heat and a combined heat+anoxia stress. A significant overlapping between the anoxic and heat shock responses has been observed by whole-genome microarray analysis. Overall design: We treated Arabidopsis seedling 4-days old dark germinated with: Control (23C dark liquid Murashige-Skoog medium containing 30mM sucrose). Heat-treated (38C for 90 minutes dark liquid Murashige-Skoog medium containing 30mM sucrose). Anoxia-treated (23C under anoxia for 6h dark liquid Murashige-Skoog medium containing 30mM sucrose). Combined heat+Anoxia-treatment (23C treated at 38C for 90 min and thereafter under anoxia for 6h dark liquid Murashige-Skoog medium containing 30mM sucrose). Two biological replicates for each condition.

Arabidopsis thaliana wild type cell cultures were exposed to a 5-day space flight onboard of Shenzhou 8 to identify microgravity and space effect related gene expression.

Articular and sterno-costal cartilages were isolated from skeletally-mature mice flown for 30 days on the BION-M1 mission. Samples were characterized histologically for proteoglycan loss and at the gene expression levels using Affymetrix gene arrays.

Spaceflight imposes the risk of skeletal muscle atrophy for astronauts. The understanding of muscle atrophy because of spaceflight is limited but continued efforts are essential for developing countermeasures of this effect. A distinct difference between spaceflight-induced muscle atrophy and other forms of atrophy is the additional effect of cosmic rays in outer space. To study spaceflight-induced muscle atrophy we performed two ground-based models of microgravity in a low dose radiation environment and studied transcriptional changes in rat soleus muscle using microarray technology.

To determine how host irradiation affects tumor profiles in 10 month aged mice treated with HZE or gamma irradiation.

Using a novel flood source construct we performed a direct comparison of genome-wide gene expression regulations resulting from exposure of primary human prostate fibroblast cultures to acute (10cGy and 200cGy) and longer-term chronic (1.0-2.45cGy cumulative over 24hr) exposures.

To investigate gene expression patterns in rat mammary carcinomas induced by ionizing radiation. Gene expression patterns were analyzed in radiation-induced rat mammary carcinomas and normal mammary gland tissues.

The present study concerns the effects of 21 days of sustained recumbence (bedrest) and hypoxia alone and in combination on skeletal muscle microRNA expression. 14 male subjects participated in 3 experimental campaigns in a counterbalanced fashion: normoxic bedrest (NBR) hypoxic bedrest (HBR) and hypoxic ambulatory confinement (HAMB) both hypoxic conditions with FO2 = 0.141 and PIO2 = 90 xc2 xb1 0.4 mmHg equivalent to an altitude of xe2 x89 x88 4000 m). Each intervention (bedrest or ambulatory confinement) lasted 21 days and the interventions were separated by a 4-month wash-out/recovery period. The order in which each subject undertook the interventions is denoted Intervention_order Throughout both bedrest interventions each subject remained in a horizontal position at all times. He was allowed one pillow for the head and to occasionally lean on an elbow while eating or being transferred to a gurney. Muscular exercise was prohibited. During the HAMB confinement each subject was allowed to move freely within the hypoxic area. To mimic a normal level of physical activity subjects performed 30 minutes of low-to-moderate-intensity exercise twice daily. Muscle samples from Vastus lateralis.

Mice were flown onboard STS-135 and returned to Earth for analysis. Cerebellums were collected within 3-4 hours of landing and snap frozen in liquid nitrogen. Samples were shipped to UCI Genomics High Throughput Facility for analysis.